Was born in Austria, and studied with Helmut Zehetmair, Corrado Romano, Ernst Kovacic and Philippe Hirschhorn.
From 1997 until 2008, he served as first Concertmaster of the Vienna Volksoper Orchestra.
In 2000, he founded the ensemble Amarcord Wien. Most of the transcriptions, which are the specificity of the ensemble, are done by members of the group.
In 2005 he joined the Hugo Wolf Quartet on the first violin.
His chamber music partners include Stefan Mendl, Markus Schirmer, Philippo Gamba, Clemens Hagen, Mario Brunello, Orfeo Mandozzi, Christian Poltera and members of the Ensemble Wien-Berlin as well as members of Art of Brass, Wolfgang Muthspiel, Allegre Corrêa, Georg Breinschmid, Kenny Wheeler, John Taylor, Aleksey Igudesman and Rebekka Bakken.
In 2016 the quartet founded in collaboration with Alban Berg Foundation, the Alban Berg Ensemble Wien, a group including stringquartet, piano, flute and clarinet. A new concept of programming and repertoire is the aim of the ensemble who is running a own serie of concert in the Brahmssaal of the Vienna Musikverein.
Sebastian Gürtler played as a soloist with the Camerata Academica Salzburg, the Orchestre de la Suisse Romande, the Vienna Chamber Orchestra, the Vienna Chamber Philharmonic, the Dresden Philharmonic, the Mozarteum Orchestra of Salzburg, the Vienna Academic Philharmonic Orchestra, the Tonkünstler Orchester of Lower Austria, under conductors such as Sandor Vegh, Lothar Zagrosek, Trevor Pinnock, Günther Herbig and Yehudi Menuhin.